INHIBITION OF INTERFERON BETA GENE TRANSCRIPTION BY THE HUMAN HERPESVIRUS 6 IMMEDIATE-EARLY 1 PROTEIN

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JOANNA JAWORSKA, ANNIE GRAVEL, KARIN FINK, NATHALIE GRANDVAUX, and LOUIS FLAMAND
Laboratory of Virology, Rheumatology and Immunology Research Center, CHUQ Research Center and Faculty of Medicine, Laval University, Quebec, Quebec, Canada; Department of Biochemistry, Faculty of Medicine, University of Montreal and CHUM Research Center-Hôpital St-Luc, Montreal, Quebec, Canada
Human Herpesviruses (HHV) are stealth pathogens possessing several decoy or immune evasion mechanisms favoring their persistence within the infected host. Of these viruses, HHV-6 is among the most successful human parasites, establishing life-long infections in nearly 100% of individuals around the world. To better understand this host-pathogen relationship, we determined whether HHV-6 could interfere with the development of the innate antiviral response by affecting interferon (IFN) biosynthesis. Using inducible cell lines and transient transfection assays, we have identified the immediate-early 1 (IE1) protein as a potent inhibitor of ifn-{beta} gene expression. IE1 from both HHV-6 variants were capable of suppressing ifn-{beta} gene induction. IE1 prevents ifn-{beta} gene expression triggered by Sendai virus (SeV) infection, double-stranded RNA (dsRNA) and dsDNA transfection or ectopic expression of ifn-{beta} gene activators such as RIG-I, MAVS, TBK-1, IKK{epsilon} or IRF3. While IFN-{beta} mRNA stability is not affected, IE1 expressing cells have reduced levels of dimerized IRF3 and nuclear translocated IRF3 in response to activation by TBK-1 or IKK{epsilon} kinases. Using nuclear extracts and gel shift experiments and we could demonstrate that in the presence of IE1, IRF3 does not bind efficiently to the IFN-{beta} promoter sequences. Overall these results indicate that the IE1 protein of HHV-6, one of the first viral proteins synthesized upon viral entry, is a potent suppressor of ifn-{beta} gene induction and likely contributes to favor the establishment and successful infection of cells by this virus.
J. Virol. doi:10.1128/JVI.02443-06


If HHV-6 is the real cause of AIDS, here are some of the implications:

1. HIV is a massive scientific fraud. Something akin to a Ponzi scheme. Scientists who challenged the HIV theory of AIDS (the ones who have been thuggishly censored and silenced) turn out to be on the money.

2. Chronic Fatigue Syndrome and Autism (and many other so-called HHV-6 related mysterious epidemics) are part of the so-called AIDS epidemic.  Chronic Fatigue Syndrome and Autism both are clearly the results of the ravages of HHV-6.

3. AIDS and Chronic Fatigue Syndrome has been artificially and politically separated into two epidemics. We are living in a period of CFS/AIDS apartheid. So-called AIDS patients have to sit in the back of the HHV-6 epidemic bus while the befuddled HHV-6/CFS patients and HHV-6/Autism victims sit up front. Nobody is well-served.

4. AIDS is not a sexually transmitted disease. That paradigm has set a scapegoating and antigay agenda in place that the public thinks is solidly based on science. It is only based on homophobic and racist nosology, epidemiology and virology. The scientists behind the paradigm are charlatans and crooks.

5. The Centers for Disease Control in Atlanta and the Pasteur Institute in Paris have a great deal in common with the institutions of Nazi medicine. For Blacks, everything these institutions have done in the name of AIDS really constitutes a second Tuskegee Syphilis Experiment.

Elements of a Scientific Ponzi Scheme like the Montagnier-Agut HIV Fraud Ponzi Scheme and HHV-6 Cover-up

A scientific Ponzi scheme begins with a central seminal or foundational scientific fraud and is  sometimes built on an infrastructure of smaller scientific frauds. Like the fake dividends issued in a strictly financial Ponzi scheme, a scientific Ponzi scheme issues fake dividends in the form of ongoing fraud-based research often framed as "breakthroughs" and bogus extrapolations which make it look like everything is above board and that what, in reality, is scientific fraud, appears to the rest of the scientific community and the public as good faith progress.

A classic scientific Ponzi scheme like the Montgnier-Agut HIV Fraud Ponzi Scheme and HHV-6 Cover-up include elements like these:

1. Nosological fraud.

2. Epidemiological fraud.

3. Virological fraud.

4. Treatment fraud.

5. Public health policy fraud.

6. Concealment of negative scientific data and paradigm-challenging anomalies.

7.  Use of an elite network of "old boys" and pseudo-activist provocateurs to censor critics and whistleblowers.

8. Chronic obscurantism.

9. If necessary, vigilantism and witch-hunts against any intellectuals, scientists, or citizens who constitute any form of resistance to the Ponzi scheme.

10. A subservient scientific press that is used as a conveyor belt for the Ponzi scheme's propaganda.

Everything always looks like it is working perfectly in a Ponzi scheme, until the moment comes when someone look at the books and blows the whistle.  Hopefully, that game-changing moment for the Montagnier-Agut HIV Fraud Ponzi Scheme and HHV-6 Cover-up is coming soon.


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