Will this also help detect African Swine Fever Virus infections in people?
Technology Could Help Detect Diseases in Commercial Swine Industry
http://www.labmanager.com/news/2015/05/technology-could-help-detect-diseases-in-commercial-swine-industry?fw1pk=2#.VVyevEab4gQ
Russian Scientist: ASF could become a human health risk
"The African swine fever (ASF) virus, may in the future become
dangerous for humans, according to the head of the Russian Epidemiology
Service, Chief State Sanitary Doctor Gennady Onishchenko, at the
press-conference in St. Petersburg. According to him almost all viruses
from time to time go through mutation processes which can give them some
additional functions."
http://www.pigprogress.net/Health-Diseases/Outbreaks/2013/7/ASF-could-become-a-human-health-risk-1308047W/
Background on African Swine Fever Virus as a human pathogen:
"African Swine fever is an endemic disease in sub-Saharan Africa and
many other parts of the developing world. It is caused by the African
Swine virus that primarily replicates in macrophages and monocytes
leading to the impairment of the structure and function of the immune
system of the infected organisms. Until now the African Swine epidemic
continues to spread despite all efforts to contain it. Thus, there is
an objective need for effective, safe and affordable preventive and
therapeutic approaches, in particular for effective vaccines, to
control and eventually eradicate this disease. Since the characteristic
feature of the African Swine virus is to impair the immune system and
to cause immune deficiencies in its hosts the development of vaccines
and other therapeutic approaches against the African Swine virus has
implications for other immune deficiencies or diseases. Several other
viruses are also known to cause immunodeficiency-like syndromes in
humans, including cytomegalovirus, Epstein Barr Virus
and others. Moreover, a series of cases of so-called "idiopathic"
immunodeficiencies have been documented that display
CD4+T-lymphocytopenia with opportunistic infections, but show no
evidence of HIV infection. Since antibodies for the African Swine virus
have been detected in humans, the possibility of human infection with
the African Swine virus exists and may thus far have escaped any systematic screening. Thus, any preventive and therapeutic approach to African Swine fever can have far-reaching implications to control immune deficiency conditions in humans."http://www.faqs.org/patents/app/20080207875
Detection of Novel Sequences Related to African Swine Fever Virus in Human Serum and Sewage.
Loh J, Zhao G, Presti RM, Holtz LR, Finkbeiner SR, Droit L, Villasana Z, Todd C, Pipas JM, Calgua B, Girones R, Wang D, Virgin HW.
Departments of Pathology & Immunology and Molecular Microbiology, Department of Medicine and Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Microbiology, Faculty of Biology, University of Barcelona, Barcelona, Spain.
"The family Asfarviridae contains only a single virus species, African swine fever virus (ASFV). ASFV is a viral agent with significant economic impact due to its devastating effects on populations of domesticated pigs during outbreaks, but has not been reported to infect humans. We report here the discovery of novel viral sequences in human serum and sewage which are clearly related to the Asfarvirus family, but highly divergent from ASFV. Detection of these sequences suggests that greater genetic diversity may exist among Asfarviruses than previously thought, and raises the possibility that human infection by Asfarviruses may occur."
http://www.ncbi.nlm.nih.gov/pubmed/19812170?dopt=AbstractDetection of Novel Sequences Related to African Swine Fever Virus in Human Serum and Sewage.
Loh J, Zhao G, Presti RM, Holtz LR, Finkbeiner SR, Droit L, Villasana Z, Todd C, Pipas JM, Calgua B, Girones R, Wang D, Virgin HW.
Departments of Pathology & Immunology and Molecular Microbiology, Department of Medicine and Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Microbiology, Faculty of Biology, University of Barcelona, Barcelona, Spain.
"The family Asfarviridae contains only a single virus species, African swine fever virus (ASFV). ASFV is a viral agent with significant economic impact due to its devastating effects on populations of domesticated pigs during outbreaks, but has not been reported to infect humans. We report here the discovery of novel viral sequences in human serum and sewage which are clearly related to the Asfarvirus family, but highly divergent from ASFV. Detection of these sequences suggests that greater genetic diversity may exist among Asfarviruses than previously thought, and raises the possibility that human infection by Asfarviruses may occur."
African Swine Fever Virus (Asfarviridae) sequences found in people with febrile illnesses
Abstract
Virus Identification in Unknown Tropical Febrile Illness Cases Using Deep Sequencing
Dengue virus is an emerging infectious agent that infects an estimated
50–100 million people annually worldwide, yet current diagnostic
practices cannot detect an etiologic pathogen in ∼40% of dengue-like
illnesses. Metagenomic approaches to pathogen detection, such as viral
microarrays and deep sequencing, are promising tools to address
emerging and non-diagnosable disease challenges. In this study, we
used the Virochip microarray and deep sequencing to characterize the
spectrum of viruses present in human sera from 123 Nicaraguan patients
presenting with dengue-like symptoms but testing negative for dengue
virus. We utilized a barcoding strategy to simultaneously deep
sequence multiple serum specimens, generating on average over 1
million reads per sample. We then implemented a stepwise bioinformatic
filtering pipeline to remove the majority of human and low-quality
sequences to improve the speed and accuracy of subsequent unbiased
database searches. By deep sequencing, we were able to detect virus
sequence in 37% (45/123) of previously negative cases. These included
13 cases with Human Herpesvirus 6 sequences. Other samples contained
sequences with similarity to sequences from viruses in the Herpesviridae, Flaviviridae, Circoviridae, Anelloviridae, Asfarviridae, and Parvoviridae
families. In some cases, the putative viral sequences were virtually
identical to known viruses, and in others they diverged, suggesting
that they may derive from novel viruses. These results demonstrate the
utility of unbiased metagenomic approaches in the detection of known
and divergent viruses in the study of tropical febrile illness.
Detection of African swine fever virus-like sequences in ponds in the Mississippi Delta through metagenomic sequencing
" . .. further study is needed to characterize their potential risks to both public health and agricultural development."http://link.springer.com/article/10.1007%2Fs11262-013-0878-2
ASF virus, adapted to grow in VERO cells, produces a strong cytopathic effect in human macrophages leading to cell destruction.
http://vir.sgmjournals.org/content/34/3/455.shortIf HHV-6 is the real cause of AIDS, here are some of the implications:
1. HIV is a massive scientific fraud. Something akin to a Ponzi scheme. Scientists who challenged the HIV theory of AIDS (the ones who have been thuggishly censored and silenced) turn out to be on the money.
2. Chronic Fatigue Syndrome and Autism (and many other so-called HHV-6 related mysterious epidemics) are part of the so-called AIDS epidemic. Chronic Fatigue Syndrome and Autism both are clearly the results of the ravages of HHV-6.
3. AIDS and Chronic Fatigue Syndrome has been artificially and politically separated into two epidemics. We are living in a period of CFS/AIDS apartheid. So-called AIDS patients have to sit in the back of the HHV-6 epidemic bus while the befuddled HHV-6/CFS patients and HHV-6/Autism victims sit up front. Nobody is well-served.
4. AIDS is not a sexually transmitted disease. That paradigm has set a scapegoating and antigay agenda in place that the public thinks is solidly based on science. It is only based on homophobic and racist nosology, epidemiology and virology. The scientists behind the paradigm are charlatans and crooks.
5. The Centers for Disease Control in Atlanta and the Pasteur Institute in Paris have a great deal in common with the institutions of Nazi medicine. For Blacks, everything these institutions have done in the name of AIDS really constitutes a second Tuskegee Syphilis Experiment.
Elements of a Scientific Ponzi Scheme like the Montagnier-Agut HIV Fraud Ponzi Scheme and HHV-6 Cover-up
A
scientific Ponzi scheme begins with a central seminal or foundational
scientific fraud and is sometimes built on an infrastructure of smaller
scientific frauds. Like the fake dividends issued in a strictly
financial Ponzi scheme, a scientific Ponzi scheme issues fake dividends
in the form of ongoing fraud-based research often framed as
"breakthroughs" and bogus extrapolations which make it look like
everything is above board and that what, in reality, is scientific
fraud, appears to the rest of the scientific community and the public as
good faith progress.
A classic scientific Ponzi scheme like the Montgnier-Agut HIV Fraud Ponzi Scheme and HHV-6 Cover-up include elements like these:
1. Nosological fraud.
2. Epidemiological fraud.
3. Virological fraud.
4. Treatment fraud.
5. Public health policy fraud.
6. Concealment of negative scientific data and paradigm-challenging anomalies.
7. Use of an elite network of "old boys" and pseudo-activist provocateurs to censor critics and whistleblowers.
8. Chronic obscurantism.
9.
If necessary, vigilantism and witch-hunts against any intellectuals,
scientists, or citizens who constitute any form of resistance to the
Ponzi scheme.
10. A subservient scientific press that is used as a conveyor belt for the Ponzi scheme's propaganda.
Everything
always looks like it is working perfectly in a Ponzi scheme, until the
moment comes when someone look at the books and blows the whistle.
Hopefully, that game-changing moment for the Montagnier-Agut HIV Fraud
Ponzi Scheme and HHV-6 Cover-up is coming soon.
The HHV-6 Rights of Man
1. The right not to be lied to about the role of HHV-6 in AIDS.
2. The right not to be lied to about the role of HHV-6 in Chronic Fatigue Syndrome.
3. The right not to be lied to about the role of HHV-6 in Autism.
4.The right not to be lied to about the role of HHV-6 in Multiple Sclerosis.
5. The right not to be lied to about the role of HHV-6 in Brain Cancer.
6. The right not to be lied to about the role of HHV-6 in Heart Disease.
7. The right not to be lied to about the role of HHV-6 in Encephalitis.
8. The right not to be lied to about the role of HHV-6 in Cognitive Dysfunction.
9. The right not to be lied to about the role of HHV-6 in Drug Hypersensitivity Syndrome.
10. The right not to be lied to about the role of HHV-6 in Bone Marrow Suppression.
11. The right not to be lied to about the role of HHV-6 in Lymphadenopathy.
12. The right not to be lied to about the role of HHV-6 in Colitis.
13. The right not to be lied to about the role of HHV-6 in Endocrine Disorders.
14. The right not to be lied to about the role of HHV-6 in Liver Disease.
15. The right not to be lied to about the role of HHV-6 in Hodgkin's Lymphoma.
16. The right not to be lied to about the role of HHV-6 in Glioma.
17. The right not to be lied to about the role of HHV-6 in Cervical Cancer.
18. The right not to be lied to about the role of HHV-6 in Hypogammaglobulinemia.
19. The right not to be lied to about the role of HHV-6 in Optic Neuritis.
20. The right not to be lied to about the role of HHV-6 in Microangiopathy.
21. The right not to be lied to about the role of HHV-6 in Mononucleosis.
22. The right not to be lied to about the role of HHV-6 in Uveitis.
23. The right not to be lied to about the role of HHV-6 in Stevens-Johnson Syndrome.
24. The right not to be lied to about the role of HHV-6 in Rhomboencephalitis.
25. The right not to be lied to about the role of HHV-6 in Limbic Encephalitis.
26. The right not to be lied to about the role of HHV-6 in Encephalomyelitis
27. The right not to be lied to about the role of HHV-6 in Pneumonitis.
28. The right not to be lied to about the role of HHV-6 in GVHD.
29. The right not to be lied to about the role of HHV-6 in Ideopathic Pneumonia.
30. The right not to be lied to about the role of HHV-6 in Pediatric Adrenocortical Tumors
31. The right not to be lied to about the role of HHV-6 in the reactivation of endogenous retroviruses.
32. The right not to be lied to about the impact of HHV-6 on T-Cells.
33. The right not to be lied to about the impact of HHV-6 on B-Cells
34. The right not to be lied to about the impact of HHV-6 on Epithelial Cells.
35. The right not to be lied to about the the impact of HHV-6 on Natural Killer Cells.
35. The right not to be lied to about the the impact of HHV-6 on Dendritic Cells.
36. The right not to be lied to about the the impact of HHV-6 infection of the brain.
37. The right not to be lied to about the the impact of HHV-6 infection of the liver.
38. The right not to lied to about the ability of HHV-6 to affect cytokine production.
39. The right not to be lied to about the ability of HHV-6 to affect Aortic and Heart Microvascular Endothelial cells.
40.
The right not to be lied to about the role of an HHV-6 cover-up in a
massive HIV Fraud Ponzi Scheme that in a number of ways resembles the
Tuskegee Syphilis Experiment and Nazi medicine.