Infection of porcine cells with human herpesviruses.

http://www.ncbi.nlm.nih.gov/pubmed/20692426

Abstract

Porcine organs are valuable candidate materials for xenotransplantation to humans. Long-term maintenance of well functioning transplants is a prerequisite for success. Transplanted organs may be damaged by immune reactions or by infectious agents in hosts. Human herpesviruses (HHVs) establish life-long latency in humans after a primary infection. They can be reactivated with various stimuli, including immunosuppression. This study was performed to verify the infectivity of some HHVs toward porcine cells. PK-15 cells infected with HHV-1 and HHV-2 showed cytopathology from 1 day after infection. Immunofluorescent (IF) staining of HHV-1- and HHV-2-infected PK-15 cells with respective antibodies demonstrated the expression of the respective viral antigens. Permissiveness of PK-15 to HHV-1 and -2 was confirmed by an infection test on Vero cells. Islet cells infected with HHV-5 showed no gross morphologic changes during the experimental course. A limited portion of islet cells reacted only to anti-IE1 and anti-IE2, but not to anti-UL44 or anti-gB antibody by IF staining, whereas a small portion of endothelial cells reacted to anti-IEs and anti-UL44, but not to anti-gB antibody. HHV-1 and -2 can permissively infect porcine cells, but HHV-5 infects a small proportion of cells with limited viral protein expression. HHV-4 could not transform peripheral blood mononuclear cells from miniature pigs. Collectively, because some HHVs can infect and damage porcine cells or impair their functions, HHVs should be cautiously monitored and controlled in humans when porcine cells or organs are transplanted to human beings.

Popular Posts in the Last 7 Days

Is Chronic Fatigue Syndrome Associated Kaposi's Sarcoma (CFSKS) a diagnosis all doctors should become aware of?

Is there, in all likelihood, an undetected epidemic of *internal* Kaposi's sarcoma in Chronic Fatigue Syndrome patients?

African Swine Fever and Kaposi's Sarcoma

Can Chronic Fatigue Syndrome patients with internal Kaposi's Sarcoma pass it on to their partners?

Bulletin #8 from the Coordinating Committee of The International HHV-6 Protest and Teach-in At Harvard (November 9-11, 2015)

HHV-6 and Thymocyte Depletion

Perplexity discusses David Ho's role in the HIV-negative case story exploded at the Amsterdam AIDS Conference in 1992

HHV-6 Linked to Miscarriages

Today Only!

Popular Post in the Last 30 Days

A Perplexity conversation about self-deceit, conspiracy, Arendt and AIDS

Groq on Hannah Arendt and science

Perplexity names the AIDS Paradigm 20

Memo to CFS patients and CFS quacks: Let's get something straight.

From Perplexity: Here are 20 ways the pharmaceutical industry should reorient their AIDS efforts based on Rebecca Culshaw’s Substack critique of the HIV paradigm:

Perplexity on the AIDS paradigm

Is Kaposi's Sarcoma in Uganda connected to the African Swine Fever epidemic in pigs?

Perplexity on K.S. and AIDS and inflammation

Perplexity on Mishmash Multifactorial Theories of CFS/AIDS

Popular Posts from the Last Year

Perplexity on Rebecca Culshaw Smith, and PrEP as Genocide

Why Brigitte Huber may be the most important AIDS and Chronic Fatigue Syndrome researcher

From Perplexity A.I.: A major nosological mistake in AIDS—meaning a fundamental error in how the disease is defined, classified, or diagnosed—could be the most consequential nosological error in history because of several deeply impactful social, scientific, and ethical consequences.

Perplexity on the issue of endogenous versus exogenous retroviruses. Is this a key to the HIV mistake?

Oral Kaposi's Sarcoma looks like the Crimson Crescents in Chronic Fatigue Syndrome patients.

Perplexity on K.S. and AIDS and inflammation

How Kaposi's Sarcoma almost undermined the HIV theory of AIDS

All Time Most Popular Posts

Dr. Bhupesh Prusty and Professor Thomas Rudel discuss their HHV-6 research

Anthony Fauci was part of the gang that silenced and destroyed Judy Mikovits.

Was Judy Mikovits destroyed because her XMRV work would have ultimately shown HIV is a total fraud?

Is Chronic Fatigue Syndrome the other AIDS epidemic in the gay community?