African Swine Fever Virus (Asfarviridae) sequences and HHV-6 found in people with febrile illnesses


Abstract
Virus Identification in Unknown Tropical Febrile Illness Cases Using Deep Sequencing
Dengue virus is an emerging infectious agent that infects an estimated 50–100 million people annually worldwide, yet current diagnostic practices cannot detect an etiologic pathogen in ∼40% of dengue-like illnesses. Metagenomic approaches to pathogen detection, such as viral microarrays and deep sequencing, are promising tools to address emerging and non-diagnosable disease challenges. In this study, we used the Virochip microarray and deep sequencing to characterize the spectrum of viruses present in human sera from 123 Nicaraguan patients presenting with dengue-like symptoms but testing negative for dengue virus. We utilized a barcoding strategy to simultaneously deep sequence multiple serum specimens, generating on average over 1 million reads per sample. We then implemented a stepwise bioinformatic filtering pipeline to remove the majority of human and low-quality sequences to improve the speed and accuracy of subsequent unbiased database searches. By deep sequencing, we were able to detect virus sequence in 37% (45/123) of previously negative cases. These included 13 cases with Human Herpesvirus 6 sequences. Other samples contained sequences with similarity to sequences from viruses in the Herpesviridae, Flaviviridae, Circoviridae, Anelloviridae, Asfarviridae, and Parvoviridae families. In some cases, the putative viral sequences were virtually identical to known viruses, and in others they diverged, suggesting that they may derive from novel viruses. These results demonstrate the utility of unbiased metagenomic approaches in the detection of known and divergent viruses in the study of tropical febrile illness.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274504/

Popular Posts in the Last 7 Days

Dr. Bhupesh Prusty and Professor Thomas Rudel discuss their HHV-6 research

More evidence HHV-6 is the cause of Chronic Fatigue Syndrome.

If you want to know the whole truth about Chronic Fatigue Syndrome, you need to go Beyond Unrest

Popular Post in the Last 30 Days

Dr. Bhupesh Prusty and Professor Thomas Rudel discuss their HHV-6 research

If you want to know the whole truth about Chronic Fatigue Syndrome, you need to go Beyond Unrest

More evidence HHV-6 is the cause of Chronic Fatigue Syndrome.

Dr. Konstance Knox explains why HHV-6 may be the key to dealing with AIDS.

Will Fauci screw up Zika as much as he has screwed up HHV-6, AIDS and Chronic Fatigue Syndrome?

Popular Posts from the Last Year

Dr. Bhupesh Prusty and Professor Thomas Rudel discuss their HHV-6 research

Oral Kaposi's Sarcoma looks like the Crimson Crescents in Chronic Fatigue Syndrome patients.

If you want to know the whole truth about Chronic Fatigue Syndrome, you need to go Beyond Unrest

More evidence HHV-6 is the cause of Chronic Fatigue Syndrome.

All Time Most Popular Posts

Dr. Bhupesh Prusty and Professor Thomas Rudel discuss their HHV-6 research

Anthony Fauci was part of the gang that silenced and destroyed Judy Mikovits.

Is Chronic Fatigue Syndrome the other AIDS epidemic in the gay community?

Was Judy Mikovits destroyed because her XMRV work would have ultimately shown HIV is a total fraud?