African Swine Fever Virus (Asfarviridae) sequences and HHV-6 found in people with febrile illnesses


Abstract
Virus Identification in Unknown Tropical Febrile Illness Cases Using Deep Sequencing
Dengue virus is an emerging infectious agent that infects an estimated 50–100 million people annually worldwide, yet current diagnostic practices cannot detect an etiologic pathogen in ∼40% of dengue-like illnesses. Metagenomic approaches to pathogen detection, such as viral microarrays and deep sequencing, are promising tools to address emerging and non-diagnosable disease challenges. In this study, we used the Virochip microarray and deep sequencing to characterize the spectrum of viruses present in human sera from 123 Nicaraguan patients presenting with dengue-like symptoms but testing negative for dengue virus. We utilized a barcoding strategy to simultaneously deep sequence multiple serum specimens, generating on average over 1 million reads per sample. We then implemented a stepwise bioinformatic filtering pipeline to remove the majority of human and low-quality sequences to improve the speed and accuracy of subsequent unbiased database searches. By deep sequencing, we were able to detect virus sequence in 37% (45/123) of previously negative cases. These included 13 cases with Human Herpesvirus 6 sequences. Other samples contained sequences with similarity to sequences from viruses in the Herpesviridae, Flaviviridae, Circoviridae, Anelloviridae, Asfarviridae, and Parvoviridae families. In some cases, the putative viral sequences were virtually identical to known viruses, and in others they diverged, suggesting that they may derive from novel viruses. These results demonstrate the utility of unbiased metagenomic approaches in the detection of known and divergent viruses in the study of tropical febrile illness.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274504/

Popular Posts in the Last 7 Days

Perplexity on multifactorialism and Peter Duesberg

New York Times: Australia has found that almost half of the sausages and other pork products carried by recently arriving passengers or the mail were contaminated

Dr. Konstance Knox explains why HHV-6 may be the key to dealing with AIDS.

A Perplexity A.I. Editorial about HHV-6

Congress needs to demand that all confiscated pork at American airports be tested for African Swine Fever virus

The false negative problem: Is there something wrong with the whole COVID-19 theory?

Is AIDS research a little too perfect?

Perplexity on Rebecca Culshaw and Chronic Fatigue Syndrome

AIDS was even worse.

Popular Post in the Last 30 Days

Perplexity on multifactorialism and Peter Duesberg

How Kaposi's Sarcoma almost undermined the HIV theory of AIDS

Has Maureen Hanson found another opportunistic infection that links Chronic Fatigue Syndrome and AIDS?

A Perplexity A.I. Editorial about HHV-6

Outbreak of COVID-19-related syndrome in children raises questions about the causation of COVID-19

Perplexity on Rebecca Culshaw and Chronic Fatigue Syndrome

Perplexity on Rebecca Culshaw's critics.

Could defining AIDS solely by HIV status create logical loops?

Suppose the real cause of AIDS is X and X is also found in Chronic Fatigue Syndrome. What are the possibilities of what X is?

Perplexity A.I. wrote this op-ed about Rebecca Culshaw's Substack

Popular Posts from the Last Year

Oral Kaposi's Sarcoma looks like the Crimson Crescents in Chronic Fatigue Syndrome patients.

Ablashi discusses HHV-6, AIDS, Alzheimer's, and Chronic Fatigue Syndrome

Why HIV should be referred to as "a red herring."

Anthony Fauci was part of the gang that silenced and destroyed Judy Mikovits.

Dr. Bhupesh Prusty and Professor Thomas Rudel discuss their HHV-6 research

All Time Most Popular Posts

Dr. Bhupesh Prusty and Professor Thomas Rudel discuss their HHV-6 research

Anthony Fauci was part of the gang that silenced and destroyed Judy Mikovits.

Was Judy Mikovits destroyed because her XMRV work would have ultimately shown HIV is a total fraud?

Is Chronic Fatigue Syndrome the other AIDS epidemic in the gay community?