Breaking News: African swine fever hits Kampala, the Capital of Uganda

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http://www.kcca.go.ug/?jsp=news&sec=91&t=African%20swine%20fever%20hits%20Kampala#.VpeIYFm8_7w

(Kampala is Uganda's national and commercial capital bordering Lake Victoria, Africa's largest lake.)

What everyone should know about African Swine Fever Virus


Russian Scientist: ASF could become a human health risk


"The African swine fever (ASF) virus, may in the future become dangerous for humans, according to the head of the Russian Epidemiology Service, Chief State Sanitary Doctor Gennady Onishchenko, at the press-conference in St. Petersburg. According to him almost all viruses from time to time go through mutation processes which can give them some additional functions."

 http://www.pigprogress.net/Health-Diseases/Outbreaks/2013/7/ASF-could-become-a-human-health-risk-1308047W/

 Was John Beldekas the first scientist to discover African Swine Fever Virus in human serum?

In August, 1986, John Beldekas was invited to go to the NCI and present his findings on the link between ASFV [African Swine Fever virus] and AIDS, which he did. Beldekas gave samples of all his lab work to Gallo. Later, the government asked Beldekas to turn over all his reagents and lab work to the government, which he did. Beldekas had found ASFV presence in nine of 21 AIDS patients using two standard procedures. At the meeting, Gallo was reported saying: “we know it is not ASFV.” How could Gallo know this as he hadn’t done any of his own tests to look for ASFV?
Two months later, Gallo published an article in Science (Oct 31, 1986) that he discovered a new possible co-factor in AIDS, a virus he called Human B Cell Lymphotropic Virus which he named HBLV. Like ASFV, HBLV infected B cells and also lived in macrophages. Did Gallo steal Beldekas’s ASF virus he found in AIDS patients and rename it HBLV? Later on, when Gallo found that HBLV could also infect other immune cells, he changed the name of HBLV to HHV-6. Eventually, Gallo identified his HBLV as the variant A strain of HHV-6 and called it a human herpesvirus.
--Mark Konlee

http://www.keephopealive.org/report10.html

Potential harm of African Swine Fever to human health is poorly undertstood
http://www.globalmeatnews.com/Industry-Markets/Russian-prisoners-fed-contaminated-pork

 



Background on African Swine Fever Virus as a human pathogen:

"African Swine fever is an endemic disease in sub-Saharan Africa and many other parts of the developing world. It is caused by the African Swine virus that primarily replicates in macrophages and monocytes leading to the impairment of the structure and function of the immune system of the infected organisms. Until now the African Swine epidemic continues to spread despite all efforts to contain it. Thus, there is an objective need for effective, safe and affordable preventive and therapeutic approaches, in particular for effective vaccines, to control and eventually eradicate this disease. Since the characteristic feature of the African Swine virus is to impair the immune system and to cause immune deficiencies in its hosts the development of vaccines and other therapeutic approaches against the African Swine virus has implications for other immune deficiencies or diseases. Several other viruses are also known to cause immunodeficiency-like syndromes in humans, including cytomegalovirus, Epstein Barr Virus and others. Moreover, a series of cases of so-called "idiopathic" immunodeficiencies have been documented that display CD4+T-lymphocytopenia with opportunistic infections, but show no evidence of HIV infection. Since antibodies for the African Swine virus have been detected in humans, the possibility of human infection with the African Swine virus exists and may thus far have escaped any systematic screening. Thus, any preventive and therapeutic approach to African Swine fever can have far-reaching implications to control immune deficiency conditions in humans."http://www.faqs.org/patents/app/20080207875

Detection of Novel Sequences Related to African Swine Fever Virus in Human Serum and Sewage.

Loh J, Zhao G, Presti RM, Holtz LR, Finkbeiner SR, Droit L, Villasana Z, Todd C, Pipas JM, Calgua B, Girones R, Wang D, Virgin HW.

Departments of Pathology & Immunology and Molecular Microbiology, Department of Medicine and Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Microbiology, Faculty of Biology, University of Barcelona, Barcelona, Spain.

"The family Asfarviridae contains only a single virus species, African swine fever virus (ASFV). ASFV is a viral agent with significant economic impact due to its devastating effects on populations of domesticated pigs during outbreaks, but has not been reported to infect humans. We report here the discovery of novel viral sequences in human serum and sewage which are clearly related to the Asfarvirus family, but highly divergent from ASFV. Detection of these sequences suggests that greater genetic diversity may exist among Asfarviruses than previously thought, and raises the possibility that human infection by Asfarviruses may occur."
http://www.ncbi.nlm.nih.gov/pubmed/19812170?dopt=Abstract

African Swine Fever Virus (Asfarviridae) sequences found in people with febrile illnesses

Abstract
Virus Identification in Unknown Tropical Febrile Illness Cases Using Deep Sequencing
Dengue virus is an emerging infectious agent that infects an estimated 50–100 million people annually worldwide, yet current diagnostic practices cannot detect an etiologic pathogen in ∼40% of dengue-like illnesses. Metagenomic approaches to pathogen detection, such as viral microarrays and deep sequencing, are promising tools to address emerging and non-diagnosable disease challenges. In this study, we used the Virochip microarray and deep sequencing to characterize the spectrum of viruses present in human sera from 123 Nicaraguan patients presenting with dengue-like symptoms but testing negative for dengue virus. We utilized a barcoding strategy to simultaneously deep sequence multiple serum specimens, generating on average over 1 million reads per sample. We then implemented a stepwise bioinformatic filtering pipeline to remove the majority of human and low-quality sequences to improve the speed and accuracy of subsequent unbiased database searches. By deep sequencing, we were able to detect virus sequence in 37% (45/123) of previously negative cases. These included 13 cases with Human Herpesvirus 6 sequences. Other samples contained sequences with similarity to sequences from viruses in the Herpesviridae, Flaviviridae, Circoviridae, Anelloviridae, Asfarviridae, and Parvoviridae families. In some cases, the putative viral sequences were virtually identical to known viruses, and in others they diverged, suggesting that they may derive from novel viruses. These results demonstrate the utility of unbiased metagenomic approaches in the detection of known and divergent viruses in the study of tropical febrile illness.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274504/

Detection of African swine fever virus-like sequences in ponds in the Mississippi Delta through metagenomic sequencing

" . .. further study is needed to characterize their potential risks to both public health and agricultural development."

http://link.springer.com/article/10.1007%2Fs11262-013-0878-2

ASF virus, adapted to grow in VERO cells, produces a strong cytopathic effect in human macrophages leading to cell destruction.

http://vir.sgmjournals.org/content/34/3/455.short

The African Swine Fever Virus Rights of Man

1. The right not to be lied to about whether African Swine Fever Virus is infecting human beings.

2. The right not to be lied to about how many different strains of African Swine Fever are capable of infecting humans.

3. The right to know if African Swine Fever is being disguised in pigs by giving it euphemisms of other diseases like PRRS and PEDV.

4. The right not to be lied to about the role of ASFV in AIDS.

5. The right not to be lied to about the role of ASFV in Chronic Fatigue Syndrome.

6. The right not to be lied to about the role of ASFV in Autism.

7.The right not to be lied to about the role of ASFV in Multiple Sclerosis.

8. The right not to be lied to about the role of ASFV in Brain Cancer.

9. The right not to be lied to about the role of ASFV in Heart Disease.

10. The right not to be lied to about the role of ASFV in Encephalitis.

11. The right not to be lied to about the role of ASFV in Cognitive Dysfunction.

12. The right not to be lied to about the role of ASFV in Drug Hypersensitivity Syndrome.

13. The right not to be lied to about the role of ASFV in Bone Marrow Suppression.

14. The right not to be lied to about the role of ASFV in Lymphadenopathy.

 15. The right not to be lied to about the role of ASFV in Colitis.

16. The right not to be lied to about the role of ASFV in Endocrine Disorders.

17. The right not to be lied to about the role of ASFV in Liver Disease.

 18. The right not to be lied to about the role of ASFV in Hodgkin's Lymphoma.

 19. The right not to be lied to about the role of ASFV in Glioma.

20. The right not to be lied to about the role of ASFV in Cervical Cancer.

21. The right not to be lied to about the role of ASFV in Hypogammaglobulinemia.

 22. The right not to be lied to about the role of ASFV in Optic Neuritis.

23. The right not to be lied to about the role of ASFV in Microangiopathy.

24. The right not to be lied to about the role of ASFV in Mononucleosis.

25. The right not to be lied to about the role of ASFV in Uveitis.

26. The right not to be lied to about the role of ASFV in Stevens-Johnson Syndrome.

27. The right not to be lied to about the role of ASFV in Rhomboencephalitis.

28. The right not to be lied to about the role of ASFV in Limbic Encephalitis.

29. The right not to be lied to about the role of ASFV in Encephalomyelitis

30. The right not to be lied to about the role of ASFV in Pneumonitis.

31. The right not to be lied to about the role of ASFV in GVHD.

32. The right not to be lied to about the role of ASFV in Ideopathic Pneumonia.

33. The right not to be lied to about the role of ASFV in Pediatric Adrenocortical Tumors

34. The right not to be lied to about the role of ASFV in the reactivation of endogenous retroviruses.

35. The right not to be lied to about the impact of ASFV on T-Cells.

36. The right not to be lied to about the impact of ASFV on B-Cells

37. The right not to be lied to about the impact of ASFV on Epithelial Cells.

38. The right not to be lied to about the the impact of ASFV on Natural Killer Cells.

39. The right not to be lied to about the the impact of HHV-6 on Dendritic Cells.

40. The right not to be lied to about the the impact of ASFV infection of the brain.

 41. The right not to be lied to about the the impact of ASFV infection of the liver.

42. The right not to lied to about the ability of ASFV to affect cytokine production.

43. The right not to be lied to about the ability of ASFV to affect Aortic and Heart Microvascular Endothelial cells.

44. The right not be lied to about the relationship of ASFV to HHV-6, HHV-7 and HHV-8. 





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