African Swine Fever Virus (Asfarviridae) sequences and HHV-6 found in people with febrile illnesses


Abstract
Virus Identification in Unknown Tropical Febrile Illness Cases Using Deep Sequencing
Dengue virus is an emerging infectious agent that infects an estimated 50–100 million people annually worldwide, yet current diagnostic practices cannot detect an etiologic pathogen in ∼40% of dengue-like illnesses. Metagenomic approaches to pathogen detection, such as viral microarrays and deep sequencing, are promising tools to address emerging and non-diagnosable disease challenges. In this study, we used the Virochip microarray and deep sequencing to characterize the spectrum of viruses present in human sera from 123 Nicaraguan patients presenting with dengue-like symptoms but testing negative for dengue virus. We utilized a barcoding strategy to simultaneously deep sequence multiple serum specimens, generating on average over 1 million reads per sample. We then implemented a stepwise bioinformatic filtering pipeline to remove the majority of human and low-quality sequences to improve the speed and accuracy of subsequent unbiased database searches. By deep sequencing, we were able to detect virus sequence in 37% (45/123) of previously negative cases. These included 13 cases with Human Herpesvirus 6 sequences. Other samples contained sequences with similarity to sequences from viruses in the Herpesviridae, Flaviviridae, Circoviridae, Anelloviridae, Asfarviridae, and Parvoviridae families. In some cases, the putative viral sequences were virtually identical to known viruses, and in others they diverged, suggesting that they may derive from novel viruses. These results demonstrate the utility of unbiased metagenomic approaches in the detection of known and divergent viruses in the study of tropical febrile illness.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274504/

Popular Posts in the Last 7 Days

Oral Kaposi's Sarcoma looks like the Crimson Crescents in Chronic Fatigue Syndrome patients.

An op-ed about CFS and AIDS written by Perplexity A.I.

Does inflammation cause the reactivation of endogenous retroviruses, some with pathological potential?

From Perplexity A.I.

Perplexity A.I. creates "Nosology Man"

Popular Post in the Last 30 Days

" A new study finds that prior herpesvirus infections are linked to a faster age-related increase in blood biomarkers associated with dementia, even in cognitively healthy women. Researchers measured five key biomarkers in 167 women and found that seropositivity for HHVs doubled the rate of biomarker accumulation compared to those without prior infection. The strongest viral associations were seen for Epstein-Barr virus and HHV6, with amyloid-beta and phosphorylated tau levels most affected. These findings support the hypothesis that chronic viral infections may accelerate neurodegeneration and raise dementia risk independently of genetic factors."

Oral Kaposi's Sarcoma looks like the Crimson Crescents in Chronic Fatigue Syndrome patients.

Pigs with ASFV also develop hypovolemia?

A satirical story about in which AIDS activists get involved in promoting and defending science that turns out to be totally wrong and backfires on them

Perplexity A.I. creates "Nosology Man"

Popular Posts from the Last Year

Oral Kaposi's Sarcoma looks like the Crimson Crescents in Chronic Fatigue Syndrome patients.

Dr. Rebecca Culshaw Smith's book is discussed on Twitter

A fictional discussion of AIDS between Senator Rand Paul and Rebecca Culshaw Smith

Ablashi discusses HHV-6, AIDS, Alzheimer's, and Chronic Fatigue Syndrome

Doctors discuss using Chloroquine for COVID-19

Is African Swine Fever a threat to human health in Congo?

All Time Most Popular Posts

Dr. Bhupesh Prusty and Professor Thomas Rudel discuss their HHV-6 research

Anthony Fauci was part of the gang that silenced and destroyed Judy Mikovits.

Was Judy Mikovits destroyed because her XMRV work would have ultimately shown HIV is a total fraud?

Is Chronic Fatigue Syndrome the other AIDS epidemic in the gay community?